［科研项目］美宝国际集团北京荣祥再生医学研究所2007年度实验研究项目
【摘要】 目的：通过细胞实验对细胞再生营养物质进行筛选，筛选出具有促进正常肺细胞生长，同时又能终止肺癌细胞生命的再生物质。方法：用6种细胞再生物质对人肺癌细胞A549和人正常胚胎肺细胞MRC5进行培养实验，在不同时间段内观察细胞的形态和生长状况，并拍照记录。结果：细胞再生物质中PRC22、PRC32可使A549细胞萎缩、稀少，直至终止，而使MRC5细胞继续存活，形态饱满，并具有增殖作用。结论：经初步筛选实验表明，细胞再生物质不仅能抑制和终止肺癌细胞的生长，而且能对正常肺细胞具有促进生长作用，但要充分论证其疗效，还要进行反复多次重复实验，并在临床实验中进行验证。
【关键词】肺癌；癌细胞；细胞再生营养物质；实验研究
【中图分类号】R734.2；R247.1【文献标识码】A【文章编号】1001-0726（2008）02-0081-05
Cells Research Report of Conquering Lung Cancer. Cells and Organs' Function Laboratory of MEBO International Group, Beijing, 100020, China
【Abstract】Objective: To screen nutrient substances of promoting growth of normal lung cell while stopping life of lung cancer cells via cell experiments. Method: Human lung cancer cells A549 and human embryonic lung cells MRC5 were cultivated with six regenerative substances of the cell. Then Cells' morphous and their growth were observed and photoed as a recording at different time points. Results: PRC22 and PRC32 of 6 regenerative substances of the cell could have cell A549 depauperate, turn into scarce and finally terminated, while could have MRC5 live continuously, keep its morphous full and proliferate. Conclusion: Primary screening experiment showed that regenerative substances of the cell not only have the function of inhibiting and stopping the growth of lung cancer cells, but also can promote the growth of normal lung cells. This still needs repeated experiments and verification in clinical studies.
【Key words】 Lung cancer； Cancer cells； Regenerative substances of the cell； Experiment study.
实验目的：通过细胞实验对细胞再生物质进行筛选，筛选出具有促进正常肺细胞生长同时又能杀灭肺癌细胞功效的再生物质。
实验品：6种再生物质实验品：PRC11，PRC21，PRC31，PRC12，PRC22，PRC32。
实验细胞：人肺癌细胞A549，人正常胚胎肺细胞MRC5
实验方法：
1.将再生物质实验品按5%浓度加至培养基中，放37℃缓释48h；
2.细胞培养：A549和MRC5 培养到一定数量分六组传代，传代后生长良好的细胞加入缓释后的培养基；
3.加入培养基后开始计时，在24h、48h、72h、5d几个时间段观察细胞的形态及生长状况，并做拍照纪录
全文PDF版浏览：
http://www.mebo.cn/07816/images/report.pdf
